About BiCell Scientific®

Antibody is a key research tool widely used in many biological experiments. It establishes the foundation of research on protein biochemistry. However, the binding affinity and specificity of antibody are the major varying factors. The majority of existing antibodies were raised against full-length protein antigens (>200 amino acids) or long-peptide (>20 amino acids) antigens. Full-length protein antigens often cause promiscuous bindings in antibody due to the large number of potential binding sites. Long peptides are very difficult to dissolve into water even in the presence of DMSO. They must be denatured prior to conjugation to carrier proteins, which is the major cause of non-specific or off-target binding.

BiCell Scientific® researchers have discovered that short-peptide antigens (13-19 amino acids) allow developing polyclonal and monoclonal antibodies with specific and bona fide binding to target proteins in their native conformation. Because the structural length of an antibody binding site spans 8 amino acids in a target protein, short-peptide antigens contain a limited number (often <10) of potential binding sites. This feature is particularly important for developing “precision” antibodies with improved binding specificity to suit high-resolution applications, such as immunohistochemistry studies that require undenatured proteins to be recognized in situ among millions of other proteins. Short antigen sequences also allow antibodies to be used in protein domain specific or amino acid site specific recognition.

BiCell Scientific® researchers have also discovered that good antibodies are determined not only by affinity but also by purity. BiCell Scientific® polyclonal antibodies are purified by dual protein G and antigen-specific affinity chromatography. BiCell Scientific® monoclonal antibodies are purified by protein G/L affinity chromatography from serum-free hybridoma culture medium.

BiCell Scientific® is dedicated to antibody manufacturing and validation. BiCell Scientific® offers a large collection of pre-validated antibodies. BiCell Scientific® also offers service to generate and validate antibodies for customers to meet the NIH guideline of authentication of key biological resources.

Poster - Site-specific polyclonal and monoclonal antibody production

Catalog - The Foundation of Biomedical Research

Monoclonal Antibody Production Flyer

Catalog - Primary Cilium

Poster - Primary Cilium Protein Antibodies

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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