Dual-color fluorescent IHC on FFPE section

FFPE sections were obtained from formalin (10% neutral formaldehyde) fixed mouse kidney tissues. Antigens on FFPE sections were retrieved by citrate solution at pH 6.0. Two BiCell Scientific antibodies, raised in rat and rabbit respectively, were incubated with FFPE sections to detect two independent antigen proteins.

Figure legend

Fluorescent IHC on mouse kidney FFPE sections shows clean and distinctive signals of two antibodies, anti-Calbindin and anti-Pendrin. Calbindin proteins are found in the cytoplasm of the principal cells in the DCT2 tubules, while Pendrin proteins are found in the luminal membrane of the intercalated cells in the DCT-CCD tubules.

FFPE section shows superb tissue morphology. Because tissues were not perfused, red blood cells are visible and may give background signals. Formalin crosslinking reaction can be effectively reversed during the antigen retrieval process, which brings back antigens in situ in tissue cells.

Figure legend

Dual-color fluorescent IHC on FFPE section clearly shows the Calbindin protein localization in the cytoplasm of the principal cells in the DCT2 tubule while the Pendrin protein localization in the luminal membrane of the intercalated cells of the same tubule segment.