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Calbindin-1 (CALB1 or Calbindin D-28K) antibody labeling of mouse cerebellum

Calbindin-1 (CALB1 or Calbindin D-28K) antibody labeling of mouse cerebellum

Calbindin-1 proteins are expressed by the Purkinje cells in the molecular layer of mouse cerebellum.

Calbindin D28K in the kidney

Calbindin D28K proteins label the distal convoluted tubules (DCT) in the mouse kidney.

Dual-color fluorescent IHC on FFPE section

FFPE section of mouse kidney was immunostained by anti-Calbindin and anti-Pendrin antibodies simultaneously and imaged by two fluorophores. Note that Calbindin antibody labels the cytoplasm of the principal cells in the DCT2 tubules, while Pendrin antibody labels the luminal membrane of the intercalated cells in the DCT2 and collecting duct tubules.

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Calbindin-1 (CALB1 or Calbindin D-28K) antibody

$155.00 – $435.00

Item Cat No.: 02401

Antibody: Rat Anti-Calbindin-1 (CALB1 or Calbindin D-28K) polyclonal antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, Mouse, Rat

Anti-Calbindin-1 (CALB1 or Calbindin D-28K) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

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Anti-Calbindin-1 (CALB1 or Calbindin D-28K) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Calbindin-1 is encoded by the CALB1 gene in human. It belongs to the calbindin family of calcium-binding proteins. Calbindin-1 consists in four active calcium-binding domains. Calbindin-1 may buffer the entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (19-aa) derived from the C-terminal region of human Calbindin-1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Dual-color fluorescent IHC on FFPE section

Fixation Techniques and Artifacts

Low-magnification fluorescent imaging Expand FFPE sections were obtained from formalin (10% neutral formaldehyde) fixed mouse kidney tissues. Antigens on FFPE sections were retrieved by citrate solution at pH 6.0. Two BiCell Scientific antibodies, raised in rat and rabbit respectively, were incubated with FFPE sections to detect two independent antigen proteins. Figure legend Fluorescent IHC on […]

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

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