The skin, or integument, consists of two major layers: the epidermis, composed of stratified squamous epithelium that is keratinized, and the dermis, composed of connective tissue.
Figure legend
E-cadherin proteins label the epithelial cell junctions in the epidermis of the skin from a mouse embryo at E12.5 on FFPE section.
The kidney is an epithelial tissue composed of the uriniferous tubules. The uriniferous tubules consists in several segments, e.g., the proximal tubule, the loop of Henle, the distal tubule and the collecting duct.
Figure legend
E-cadherin proteins label the epithelial cell junctions in the uriniferous tubules of the kidney from a mouse embryo at E12.5 on FFPE section.
The gut is lined with simple columnar epithelium, which serves two main functions: absorbing nutrients and restricting the entry of harmful substances. As part of its protective role, the gut epithelium forms a mucosal barrier.
Figure legend
E-cadherin proteins label the epithelial cell junctions of the mucosa in the gut from a mouse embryo at E12.5 on FFPE section.
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Item Cat No.: 00101
Antibody: Rabbit E-cadherin (Cdh1 or CD324) Polyclonal Antibody
Concentration: 0.25 mg/ml purified IgG
Application: Validated by immunofluorescence labeling (1:100)
Reactivity: Human, mouse, rat
Read More"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."
Jerrold R. Turner, M.D., Ph.D.
"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."
Univ.-Prof. Jay Gopalakrishnan PhD
"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."
Julie Craft Van De Weghe, PhD
"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"
Divyesh Joshi, PhD
I have tested the rat polyclonal IgGs to ABCD1 by immunoflourescence on cells overexpressing ABCD1. The antibodies successfully detected the protein (either untagged or tagged with GFP) at a 1:500 dilution and there was little background. The antibodies did not detect ABCD2. So this is very good news, and you may now go ahead and clone out a monoclonal!
Annette Ehrhardt, PhD
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