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Custom Lentivirus Production

$2,150.00

Item Cat No.: BCFTLV

Application: Custom Lentivirus Production

Reactivity: Cell culture

BiCell Scientific’s lentivirus production service provides customized lentivirus design and packaging services including lentiviral vector cloning, lentivirus generation, packaging and titration, target cell transduction and transgenic expression validation.

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Lentivirus is a genus of retroviruses, which includes the human immunodeficiency virus (HIV). Lentiviruses offer many unique advantages over other conventional and viral gene-delivery systems. Principal among these is their ability to provide long-term and stable gene expression and to transduce both dividing and non-dividing cells, such as neurons, T cells, and even early-stage embryos. Compared to retroviruses, lentiviruses are less likely integrated into heterochromatin where transgenes are tightly packed and silenced by epigenetic mechanisms.

As with all retroviruses, lentiviruses have gag, pol and env genes, coding for viral proteins in the order: 5´-gagpolenv-3´. Unlike other retroviruses, however, lentiviruses have two regulatory genes, tat and rev. The Long terminal repeat (LTR) is about 600 nt long, of which the U3 region is 450, the R sequence 100 and the U5 region some 70 nt long.

BiCell Scientific’s lentivirus production service provides customized lentivirus design and packaging services including lentiviral vector cloning, lentivirus generation, packaging and titration, target cell transduction and transgenic expression validation.

  • Molecular cloning of transgene into lentiviral backbone vector
  • Transfection of HEK293T cells with lentiviral backbone vector as well as vectors expressing gagpol, rev and vsvg
  • Harvesting and purification of lentivirus from cell culture medium
  • Transducing target cells and titrating virus titer with GFP expression
  • Validation of transgene expression in target cells with IF/IHC
  • Microscopic imaging of transgene expression in target cells

Customers will receive the following deliverables at the end of each project:

  • Validated Lentiviral backbone vector (3rd generation) containing desired transgene
  • Validated pre-packaged lentivirus of 1ml at titer of 1×109/ml pseudotyped with VSV-G coat protein and able to transduce target cells at titer of 1×106/ml
  • Validation of target cell transduction at >90% with GFP expression
  • Validation of transgenic expression in target cells with IF/IHC
  • Lentiviral vector cloning – 2-3 weeks
  • Lentivirus packaging  – 1-2 weeks
  • Target cell transduction – 1-2 weeks
  • Transgenic expression validation – 2-3 weeks

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

Contact us for questions or custom requests!