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Endo-binder Fluorophore Conjugate

$175.00

Concentration: 0.1 mg/ml

Application: Labeling endosome and lysosome

Reactivity: Cell culture

200 µl size

Endo-binder Fluorophore Conjugate labels the acidic vesicles within a cell including the endosomes and the lysosomes.

BiCell Scientific Inc’s Endo-binder is a type of pH-sensitive lipid-permeable fluorophore that stains acidic compartments within a cell. Endo-binder consist of a hydrophobic fluorophore linked to a weak base that is only partially protonated at neutral pH. In a neutral pH, Endo-binder can freely diffuse across the plasma membranes of live cells. Once entering the endosome or the lysosome, the basic moiety is protonated, which prevents the fluorophore from diffusing across the organelle membrane, therefore providing a localized accumulation for distinct labeling of acidic organelles.

Endo-binder is suitable for labeling live cells as well as fixed cells but not suitable for labeling histological tissue section.

Conjugation: Fluorophore conjugated

Purification: HPLC

Storage buffer: DMSO

Storage condition: –20°C


For Research Use Only. Not for use in clinical diagnostics.

Staining of cell culture

  • Dilute Endo-binder Fluorophore Conjugate at 1:100 in PBS or cell culture medium.
  • Add diluted Endo-binder to cell culture and incubate for 1hr at 37oC.
  • Aspirate cell culture medium and fix cells in 3.7% PFA for 1hr at 4oC.
  • Image labeled cells with fluorescent microscopy.

Additional information

Fluorescent labels

Green Fluorophore, Red Fluorophore

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

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