Endo-binder Fluorophore Conjugate

BiCell Scientific Inc’s Endo-binder is a type of pH-sensitive lipid-permeable fluorophore that stains acidic compartments within a cell. Endo-binder consist of a hydrophobic fluorophore linked to a weak base that is only partially protonated at neutral pH. In a neutral pH, Endo-binder can freely diffuse across the plasma membranes of live cells. Once entering the endosome or the lysosome, the basic moiety is protonated, which prevents the fluorophore from diffusing across the organelle membrane, therefore providing a localized accumulation for distinct labeling of acidic organelles.

Endo-binder is suitable for labeling live cells as well as fixed cells but not suitable for labeling histological tissue section.