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Non-aggregating High-resolution Secondary Antibody ImmunoGold Conjugate

$475.00

Antibody: Secondary Polyclonal Antibody

Concentration: 0.50 mg/ml

Application: Electron Microscopy

Reactivity: Rabbit, Rat, Mouse

200 µl size

Non-aggregating High-resolution Secondary Antibody ImmunoGold Conjugate allows colloidal gold nanoparticle labeling of primary polyclonal or monoclonal antibodies.

Immunogold conjugated secondary antibodies allow location studies of target proteins at nanometer scales with electron microscopic techniques.

The electron-dense nature of colloidal gold nanoparticles makes them ideal “marker” molecules for electron microscopy. Double labeling can be performed by combining two sizes of particle, their sizes distinguishable by electron microscopy allowing differentiation of two target proteins below the diffraction limit of light.

BiCell Scientific Inc has developed a proprietary conjugation approach that is able to maintain the solubility of gold nanoparticle conjugated antibodies in the colloidal state, thus minimizing the aggregation of secondary antibody molecules and reducing the background signals on electron micrographs.

Host/Isotype: Goat/IgG

Class: Polyclonal

Immunogen: Target species IgG (H+L)

Conjugation: Immunogold

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C


For Research Use Only. Not for use in clinical diagnostics.

  • Fix tissues using appropriate fixative (e.g. 10% formalin or 4% PFA) (avoid glutaraldehyde in immunoEM application).
  • Dehydrate tissues with increasing concentrations of ethyl alcohol (50% to 100%).
  • Embed tissues in LR Gold resin.
  • Section tissues (70nm ultrathin sections) with ultramicrotome.
  • Transfer sections to formvar-carbon coated copper grids. Air-dry the grids.
  • Rehydrate sections with decreasing concentrations of ethyl alcohol (100% to 70%).
  • Wash grids 2 times with dH2O.
  • Dip grids into universal antigen retrieval buffer.
  • Microwave sections at boiling temperature for 10min.
  • Wash grids 2 times with dH2O.
  • Drain Wash solution against a towel and float grids on drops of Block solution.
  • Transfer grids to 5 µl drops of primary antibody diluted at 1:50 in Block solution.
  • Incubate grids at room temperature for 2hr.
  • Drain grids off antibody, and wash three times with the Wash solution.
  • Transfer grids to 5 µl drops of immunogold conjugated secondary antibody diluted at 1:50 in Block solution.
  • Incubate grids at room temperature for 1hr.
  • Drain grids off antibody, and wash three times with the Wash solution.
  • Contrast grids in uranyl acetate.

Additional information

Target species

Rabbit, Rat, Mouse

Immunogold conjugates

5 nm Gold Nanoparticle, 10 nm Gold Nanoparticle, 20 nm Gold Nanoparticle, 40 nm Gold Nanoparticle

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Be the first to review “Non-aggregating High-resolution Secondary Antibody ImmunoGold Conjugate”

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

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