Non-aggregating High-resolution Secondary Antibody ImmunoGold Conjugate

Immunogold conjugated secondary antibodies allow location studies of target proteins at nanometer scales with electron microscopic techniques.

The electron-dense nature of colloidal gold nanoparticles makes them ideal “marker” molecules for electron microscopy. Double labeling can be performed by combining two sizes of particle, their sizes distinguishable by electron microscopy allowing differentiation of two target proteins below the diffraction limit of light.

BiCell Scientific Inc has developed a proprietary conjugation approach that is able to maintain the solubility of gold nanoparticle conjugated antibodies in the colloidal state, thus minimizing the aggregation of secondary antibody molecules and reducing the background signals on electron micrographs.