Shop Antibodies

Sandwich ELISA development

Four high affinity clones were selected for sandwich ELISA development and manufactured to milligram level. S1D1E5 was used as detection antibody and conjugated to HRP, and three other clones including S1D2C8 were used as capture antibody and coated to plastic plate.

Omicron mutation effect on antibody binding

Four mAb clones were tested for binding difference between wild type (WT) S1 protein and Omicron variant S1 protein. One clone: S1P2B5 was affected by Omicron mutations. S1D2C8 and S1D1E5 were largely unaffected by Omicron mutations.

SARS-CoV-2 (Covid-19) ELISA Kit

$850.00

Item Cat No.: ELICV19

Application: ELISA

Reactivity: Covid-19 virus, S1 protein

1 kit (96 samples)

SARS-CoV-2 (Covid-19) ELISA Kit allows quantitative detection of SARS-CoV-2 (Covid-19) viruses in biological samples.

SARS-CoV-2 (Covid-19) ELISA Kit is established upon two validated monoclonal antibodies (S1D2C8 and S1D1E5) raised against the spike S1 protein of Covid-19. This kit has been validated with recombinant S1 protein as well as heat-inactivated Covid-19 virus from ATCC.

Additional monoclonal antibodies against Covid-19 are available here: MABCV19.

 

For Research Use Only. Not for use in clinical diagnostics.

The kit contains:

  • Capture antibody solution: S1D2C8
  • Detection antibody solution: S1D1E5-HRP
  • Recombinant S1 protein (100 ug/ml)
  • ELISA reaction buffer
  • 96-well plate
  • HRP substrate
  • Stop solution

Store at 4oC

  • Coat the 96-well plate by adding 100 ul of Capture antibody to each well and incubate the plate at 4oC for overnight
  • Wash the plate with ELISA reaction buffer 3 times
  • Make S1 protein standards (100, 10, 1, 0.1, 0.01, and 0.001 ug/ml) by doing serial 1:10 dilutions of Recombinant S1 protein with ELISA reaction buffer
  • Add 100 ul of each S1 standard into 96-well plate
  • Dilute virus samples with ELISA reaction buffer
  • Add 100 ul of each virus sample into 96-well plate
  • Incubate the 96-well plate for 30 min at room temperature with gentle shaking.
  • Wash the plate with ELISA reaction buffer 3 times
  • Add 100 ul of Detection antibody solution to each well
  • Incubate the 96-well plate for 30 min at room temperature with gentle shaking.
  • Wash the plate with ELISA reaction buffer 3 times
  • Add 100 ul of HRP substrate to each well
  • Incubate the 96-well plate for <5 min at room temperature with gentle shaking until blue color develops.
  • Add 50 ul of Stop solution to each well
  • OD450nm

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Julie Craft Van De Weghe, PhD

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"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

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