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SARS-CoV-2 (Covid-19) ELISA Kit


Item Cat No.: ELICV19

Application: ELISA

Reactivity: Covid-19 virus, S1 protein

1 kit (96 samples)

SARS-CoV-2 (Covid-19) ELISA Kit allows quantitative detection of SARS-CoV-2 (Covid-19) viruses in biological samples.

SARS-CoV-2 (Covid-19) ELISA Kit is established upon two validated monoclonal antibodies (S1D2C8 and S1D1E5) raised against the spike S1 protein of Covid-19. This kit has been validated with recombinant S1 protein as well as heat-inactivated Covid-19 virus from ATCC.

Additional monoclonal antibodies against Covid-19 are available here: MABCV19.


For Research Use Only. Not for use in clinical diagnostics.

The kit contains:

  • Capture antibody solution: S1D2C8
  • Detection antibody solution: S1D1E5-HRP
  • Recombinant S1 protein (100 ug/ml)
  • ELISA reaction buffer
  • 96-well plate
  • HRP substrate
  • Stop solution

Store at 4oC

  • Coat the 96-well plate by adding 100 ul of Capture antibody to each well and incubate the plate at 4oC for overnight
  • Wash the plate with ELISA reaction buffer 3 times
  • Make S1 protein standards (100, 10, 1, 0.1, 0.01, and 0.001 ug/ml) by doing serial 1:10 dilutions of Recombinant S1 protein with ELISA reaction buffer
  • Add 100 ul of each S1 standard into 96-well plate
  • Dilute virus samples with ELISA reaction buffer
  • Add 100 ul of each virus sample into 96-well plate
  • Incubate the 96-well plate for 30 min at room temperature with gentle shaking.
  • Wash the plate with ELISA reaction buffer 3 times
  • Add 100 ul of Detection antibody solution to each well
  • Incubate the 96-well plate for 30 min at room temperature with gentle shaking.
  • Wash the plate with ELISA reaction buffer 3 times
  • Add 100 ul of HRP substrate to each well
  • Incubate the 96-well plate for <5 min at room temperature with gentle shaking until blue color develops.
  • Add 50 ul of Stop solution to each well
  • OD450nm


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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