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Antibody Drug Conjugation (ADC)


Item Cat No.: BCADCL

Antibody: Monoclonal Antibody

Concentration: 1 mg/ml of purified IgG

Application: ADC Cytotoxicity

Reactivity: Human, Mouse, Rat

BiCell Scientific Inc has developed a robust bioconjugation protocol to add a variety of small molecules to monoclonal antibodies as well as biosimilar antibodies.

1 mg scale

Antibody–drug conjugate (ADC) is composed of a monoclonal antibody covalently attached to a cytotoxic drug via a chemical linker. It combines both the advantages of highly specific targeting ability of an antibody and highly potent killing effect of a small chemical to achieve accurate and efficient elimination of tumor cells.

There are two major categories of conjugation approaches. (1) Conjugation on ε-amino group via an active carboxylic acid ester; (2) Conjugation on reduced cysteine group via maleimide. An antibody protein can contain up to 90 lysine residues, of which 40 lysine residues are modifiable. Through the random coupling, varying numbers (0–8) of small-molecule toxins may be conjugated to an antibody, resulting in a wide drug-antibody ratio (DAR) distribution. Due to the limited number of cysteine sites, and therefore a DAR ratio of 1-4, conjugation on cysteine group can reduce the heterogeneity of ADC. However, opening the disulfide bond via reduction may alter the integrity of antibody protein.

BiCell Scientific’s ADC service includes two analytic chromatographs, using hydrophobic column and gel filtration column, which reveal the ADC effect on antibody aggregation and molecular size variation.

Customers will receive the following deliverables at the end of each project:

  • 1 mg of drug-conjugated and affinity-purified monoclonal antibody or biosimilar antibody
  • analytic chromatograph of gel filtration column
  • analytic chromatograph of hydrophobic column

For Research Use Only. Not for use in clinical diagnostics.

Additional information

Conjugation Site

Lysine, Cysteine

Chemical Payload

Auristatin, Maytansinoid, Tubulysin, Calicheamicin, Duocarmycin, Exatecan, Pyrrolobenzodiazepine, STING agonist, Custom Drug


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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