Formalin is a widely used fixative for tissue preservation in histology and pathology. Autofluorescence frequently hampers visualization of immunofluorescent markers in formalin-fixed paraffin-embedded (FFPE) tissues. Key sources of autofluorescence in FFPE tissues are lipofuscin, collagen, elastin, and red blood cells.
FFPE autofluorescence quenching buffer, derived from Sudan Black biological stain, is able to bind to a variety of lipids and quench autofluorescence from lipofuscin and red blood cells.
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