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Hoechst Blue Stain labeling of mouse embryo

Hoechst Blue Stain labeling of mouse embryo

Hoechst Blue Stain labels the nuclei in the dorsal root ganglion of a mouse embryo at E12.5.

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Hoechst Blue Stain

$125.00

Item Cat No.: 00001

Concentration: 1 mg/ml

Application: IF/IHC on Cryosection and FFPE

Reactivity: Tissue

200 µl size

Hoechst Blue Stain labels the chromosomal DNA in the nuclei of rodent and human cells.

Hoechst stains are a family of blue fluorescent dyes that bind to the minor groove of the DNA double helix and are able to recognize a run of four A-T base pairs.

Hoechst stains are excited by ultraviolet light at 350 nm, and emit blue fluorescent light at 460 nm.

This stain contains a mixture of Hoechst dyes. The recipe of the stain is proprietary.

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

  • Deparaffinize FFPE sections with Xylene.
  • Rehydrate sections with decreasing concentrations of ethyl alcohol (100% to 70%).
  • Wash slides 2 times with dH2O.
  • Dip slides into universal antigen retrieval buffer.
  • Microwave sections at boiling temperature for 10min.
  • Wash slides 2 times with dH2O.
  • Dip slides into FFPE autofluorescence quenching buffer for 20min.
  • Wash slides with 70% ethyl alcohol
  • Wash slides with 1xPBS
  • Drain Wash solution against a towel and put slides into the wet chamber.
  • Dilute Hoechst Blue Stain at 1:100 in Block solution and add to each slide (300-500 µl is sufficient to cover the section on each slide).
  • Incubate the slides in the wet chamber at room temperature for 1hr.
  • Take slides out of the wet chamber, drain off Hoechst Blue Stain, and wash three times with the Wash solution.
  • Add 20 ul of Mowiol solution to the section on each slide. Place a cover glass onto the section and apply a small pressure to remove any trapped air bubble. Let Mowiol solution dry at room temperature for 1hr. Store glass slides at 4oC for less than 1 month.

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