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Custom CAR-T Cell

$29,750.00

Item Cat No.: BCFVCAR

Application: CAR-T cell development

Reactivity: Cell culture

BiCell Scientific’s CAR-T Cell Service provides customized pre-clinical CAR-T Cell generation and validation services including monoclonal antibody development, chimeric antigen receptor cloning, lentivirus packaging, mouse T cell transduction, and in vitro testing of CAR-T cells.

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Chimeric antigen receptor T cells (also known as CAR T cells) are T cells that have been genetically engineered to produce an artificial T cell receptor for use in immunotherapy.

Chimeric antigen receptors (CARs, also known as chimeric immunoreceptors, chimeric T cell receptors or artificial T cell receptors) are artificial receptor proteins that have been genetically engineered to give T cells the new ability to target a specific protein on tumor cells. The receptors are chimeric because they combine the antigen-binding domain of a monoclonal antibody and the T cell activating domain of CD3ζ protein into a single receptor.

BiCell Scientific’s CAR-T Cell Service provides customized pre-clinical CAR-T Cell generation and validation services including monoclonal antibody development, chimeric antigen receptor cloning, lentivirus packaging, mouse T cell transduction, and in vitro testing of CAR-T cells.

  • Mouse, rat or rabbit hybridoma selection against short peptide antigen
  • Monoclonal antibody production and validation
  • Molecular cloning of monoclonal antibody cDNA
  • Molecular cloning of single-chain variable fragment (scFv) of heavy and light chains of antibody
  • Molecular assembly of chimeric antigen receptor with scFv domain, CD8α transmembrane domain, CD3ζ cytoplasmic signaling domain, and CD28 costimulatory molecule
  • Molecular packaging of assembled CAR protein into lentivirus
  • Transduction of mouse T cells with lentivirus
  • Validation of CAR binding in T cells with fluorophore labeled peptide antigen
  • In vitro assay of the cytotoxic effect of CAR-T cells on tumor cells or cultured cells expressing antigen proteins

Customers will receive the following deliverables at the end of each project:

  • Validated hybridoma cell line with guaranteed monoclonal antibody binding affinity and specificity
  • Validated scFv cDNA sequence with guaranteed folding stability
  • Fully assembled CAR protein expression vector cloned into lentiviral backbone
  • Validated pre-packaged lentivirus pseudotyped with VSV-G coat protein and able to transduce mouse T cells at titer of 1×107
  • Guaranteed cytotoxic effects of CAR-T cells on tumor cells or cultured cells expressing antigen proteins
  • Monoclonal antibody production – 3-4 months
  • Molecular cloning of chimeric antigen receptor – 1 month
  • Lentivirus packaging and transduction of mouse T cells – 1 month
  • In vitro cytotoxic testing of CAR-T cells – 1 month

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Be the first to review “Custom CAR-T Cell”

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

Contact us for questions or custom requests!