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Lotus Tetragonolobus Lectin (LTL) FITC Conjugate labeling of mouse kidney

Lotus Tetragonolobus Lectin (LTL) FITC Conjugate labeling of mouse kidney

LTL labels the brush border of the luminal membrane of the proximal tubule while Aqp2 antibody labels the luminal membrane of the distal tubule in the mouse kidney.

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Lotus Tetragonolobus Lectin (LTL) Fluorophore Conjugate

$175.00

Concentration: 1 mg/ml

Application: IF/IHC on Cryosection and FFPE

Reactivity: Tissue

200 µl size

Lotus Tetragonolobus Lectin (LTL) Fluorophore Conjugate labels the brush border of the proximal tubule in the kidney.

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Lotus Tetragonolobus Lectin (LTL) is a family of closely related glycoproteins that show binding affinities and specificities for oligosaccharides present in cellular to subcellular structures, e.g. the brush border of the proximal tubule in the kidney. Lotus Tetragonolobus Lectin (LTL) Fluorophore Conjugate can be used to label the brush border of the proximal tubule in the kidney.

Conjugation: Fluorophore conjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

  • Deparaffinize FFPE sections with Xylene.
  • Rehydrate sections with decreasing concentrations of ethyl alcohol (100% to 70%).
  • Wash slides 2 times with dH2O.
  • Dip slides into universal antigen retrieval buffer.
  • Microwave sections at boiling temperature for 10min.
  • Wash slides 2 times with dH2O.
  • Dip slides into FFPE autofluorescence quenching buffer for 20min.
  • Wash slides with 70% ethyl alcohol
  • Wash slides with 1xPBS
  • Drain Wash solution against a towel and put slides into the wet chamber.
  • Dilute Lotus Tetragonolobus Lectin (LTL) Fluorophore Conjugate at 1:100 in Block solution and add to each slide (300-500 µl is sufficient to cover the section on each slide).
  • Incubate the slides in the wet chamber at room temperature for 1hr.
  • Take slides out of the wet chamber, drain off LTL labels, and wash three times with the Wash solution.
  • Add 20 ul of Mowiol solution to the section on each slide. Place a cover glass onto the section and apply a small pressure to remove any trapped air bubble. Let Mowiol solution dry at room temperature for 1hr. Store glass slides at 4oC for less than 1 month.

Additional information

Fluorescent labels

FITC, Rhodamine

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