Shop Antibodies

Custom MicroRNA Expression with Retrovirus & Lentivirus


Item Cat No.: BCFTMI

Application: Gene silencing

Reactivity: Cell culture

BiCell Scientific’s microRNA expression service provides pre-packaged and ready-to-use retrovirus or lentivirus to deliver microRNA molecules for gene silencing.

Product total
Options total
Grand total

MicroRNA (miRNA) are small, single-stranded, non-coding RNA molecules containing 21 to 23 nucleotides. MicroRNAs resemble the small interfering RNAs (siRNAs) of the RNA interference (RNAi) pathway, except that microRNAs derive from regions of RNA transcripts that fold back on themselves to form short hairpins, whereas siRNAs derive from longer regions of double-stranded RNA. In cells of humans and other mammals, microRNAs silence gene expression primarily by destabilizing the mRNA whereas siRNAs cleaving the mRNA.

MicroRNA genes are transcribed by RNA polymerase II (Pol II) while siRNAs are transcribed by RNA polymerase III (Pol III). MicroRNA transcript is capped with a specially modified nucleotide at the 5′ end, and polyadenylated with multiple adenosines into a poly(A) tail.

BiCell Scientific’s microRNA expression service provides customized microRNA gene cloning and packaging services into retrovirus or lentivirus. The pre-packaged and ready-to-use retrovirus or lentivirus can infect a variety of mammalian cells to induce microRNA-dependent gene silencing.

  • Design and cloning of microRNA minigene into retroviral or lentiviral backbone vector
  • Transfection of HEK293T cells with retroviral or lentiviral backbone vector as well as vectors expressing gagpol, rev and vsvg
  • Harvesting and purification of virus from cell culture medium
  • Transducing target cells and titrating virus titer with GFP expression
  • Validation of gene silencing in target cells with IF/IHC

Customers will receive the following deliverables at the end of each project:

  • Validated microRNA expressing vector in retroviral or lentiviral backbone plasmid
  • Validated pre-packaged retrovirus or lentivirus of 1-ml at titer of 1×109/ml pseudotyped with VSV-G coat protein and able to transduce target cells at titer of 1×106/ml
  • Validation of target cell transduction at >90% with GFP expression
  • Validation of gene silencing in target cells with IF/IHC
  • Design and cloning of microRNA minigene – 2-3 weeks
  • Retrovirus or lentivirus packaging  – 1-2 weeks
  • Target cell transduction – 1-2 weeks
  • Gene silencing validation – 2-3 weeks

Additional information

Carrier virus

Retrovirus, Lentivirus


There are no reviews yet.

Be the first to review “Custom MicroRNA Expression with Retrovirus & Lentivirus”

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

Contact us for questions or custom requests!