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GFP Nanobody Production Strategy

GFP Nanobody Production Strategy

Top panel: the plasmid diagram showing the mammalian/bacterial dual expression vector for GFP nanobody. Bottom panel: the amino acid sequences of GFP nanobody.

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GFP Nanobody

$205.00 – $2,500.00

Item Cat No.: NB0001

Antibody: Nanobody

Concentration: 1 mg/ml of purified VHH fragment

Application: In vitro and In vivo

Reactivity: Human, mouse, rat

The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody developed with specific binding affinity to GFP.

The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody developed with specific binding affinity to GFP and is emerging as a powerful tool for isolation and cellular engineering of fluorescent protein fusions in many biological fields.

BiCell Scientific Inc utilizes a published GFP nanobody sequence (J Cell Sci. 2015) to manufacture GFP nanobody proteins. BiCell Scientific Inc offers both unconjugated GFP nanobody and various conjugated forms of GFP nanobody to facilitate cellular localizing, tracing and functional studies.

Host/Isotype: Alpaca (Lama pacos)

Class: Nanobody

Immunogen: GFP

Production platform: E.coli

Purification: Nickel affinity chromatography

Storage buffer: PBS, pH 7.2

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics or therapeutics.

Additional information

Antibody Production Scale

50 ug, 250 ug, 1 mg
Conjugate

None, FITC, HRP, ADC (custom drug)

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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