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NHE3 (Slc9a3) (rat) antibody


Item Cat No.: 21003rs

Antibody: Rat NHE3 (Slc9a3) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-NHE3 (Slc9a3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

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Anti-NHE3 (Slc9a3) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Sodium-hydrogen exchanger 3 (NHE3), or sodium-hydrogen antiporter 3, is encoded by the SLC9A3 gene in human. NHE3 is an epithelial brush border Na/H exchanger that uses an inward sodium ion gradient to expel acids from the cell.

NHE3 consists in 10 transmembrane domains. NHE3 is found on the luminal membrane of the epithelial cells of the proximal tubule in the kidney and the epithelial cells in the intestinal mucosa.

Mutations in NHE3 are a cause of congenital secretory sodium diarrhea (OMIM 616868).

This antibody is raised in rat and particularly suited for use as a molecular marker to label the proximal tubules in co-localization studies.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the C-terminal cytoplasmic region of mouse NHE3 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 62.5% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

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