Site-specific changes in protein vital for diseases are hard to detect with existing methods, such as point mutations in cancer proteins or Alzheimer’s proteins underlying disease progression. Point modifications in proteins, such as phosphorylation, acetylation, etc are often overlooked in disease diagnostics due to the lack of reliable antibodies.
BiCell Scientific Inc researchers have discovered that short-peptide antigens (13-19 amino acids) harboring single site mutation or modification allow developing polyclonal and monoclonal antibodies with specific binding to the mutated or modified site in a target protein. BiCell Scientific Inc has introduced the depletion chromatography technique with affinity column conjugated to a peptide carrying the same sequence as the antigen peptide, but not the mutation or modification, to remove unwanted bindings of antibody as an additional measure to ensure site specificity.
Protein modifications that can be targeted for site specific polyclonal or monoclonal antibody production include:
– Acetylation; Cleavage sites; Drug binding Isoforms; Glycosylation; Ligand binding; Myristolation;
– Phosphorylation; Prenylation; Splice variants; Sumoylation; Ubiquitination; Mutations/Polymorphisms.
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