Name: Universal antigen retrieval buffer
SKU: BCASUB
Availability: InStock

Universal antigen retrieval buffer

$155.00 – $375.00

Item Cat No.: BCASUB

Application: IHC on FFPE

Reactivity: Tissue

Universal antigen retrieval buffer allows antigen retrieval from FFPE sections and immunolabeling with antibodies for IHC purposes.

Formalin is a widely used fixative for tissue preservation in histology and pathology. Formalin fixed tissues are difficult to label with antibodies largely because formalin conceals the antigens by protein cross-linking.

Universal antigen retrieval buffer utilizes the ability of citraconic group to hydrolyze the adducts of primary amines that are cross-linked by formalin, and therefore liberate the cross-linked proteins.

Universal antigen retrieval buffer can retrieve approximately 10-time more antigen signals than conventional antigen retrieval buffers, such as acidic citrate buffer or alkaline Tris buffer.

This buffer contains citraconic derivatives and other substances. The recipe of the buffer is proprietary.

Storage condition: 4°C

For Research Use Only. Not for use in clinical diagnostics.

Deparaffinize FFPE sections with Xylene.
Rehydrate sections with decreasing concentrations of ethyl alcohol (100% to 70%).
Wash slides 2 times with dH₂O.
Dip slides into universal antigen retrieval buffer.
Microwave sections at boiling temperature for 10min.
Wash slides 2 times with dH₂O.
Drain Wash solution against a towel and put slides into the wet chamber.
Dilute primary antibody at 1:50 in Block solution and add to each slide (300-500 µl is sufficient to cover the section on each slide).
Incubate the slides in the wet chamber at room temperature for 2hr.
Take slides out of the wet chamber, drain off antibody, and wash three times with the Wash solution.
Dilute secondary antibodies at 1: 200 in Block solution and add to each slide and put slides back into the wet chamber.
Incubate the slides in the wet chamber at room temperature for 1hr.
Take slides out of the wet chamber, drain off antibody, and wash three times with the Wash solution.
If using fluorophore labeled secondary antibodies, then add 20 ul of Mowiol solution to the section on each slide. Place a cover glass onto the section and apply a small pressure to remove any trapped air bubble. Let Mowiol solution dry at room temperature for 1hr. Store glass slides at 4^oC for less than 1 month.
If using HRP labeled secondary antibodies, then add HRP substrates such as DAB and follow the instructions of DAB colorimetric development.

Additional information

Volume	1 Liter, 1 Gallon

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