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BiCellFectamax Transfection Reagent

$185.00$2,050.00

Item Cat No.: T0010

Application: Cell Transfection

Reactivity: Cell culture

BiCellFectamax Transfection Reagent offers improved cell transfection efficiency by modifying liposome surface chemistry with polyethylene glycol (PEG).

Liposome is a common transfection approach used to transfect DNA or RNA into in vitro cell cultures by lipofection. Liposome entraps the transfected payload, e.g. DNA or RNA in an aqueous environment.

Common liposome reagents consist of a 3:1 mixture of DOSPA (2,3‐dioleoyloxy‐N‐ [2(sperminecarboxamido)ethyl]‐N,N‐dimethyl‐1‐propaniminium trifluoroacetate) and DOPE (1,2-Dioleoyl-sn-glycerophosphoethanolamine), which complexes with negatively charged nucleic acid molecules to allow them to overcome the electrostatic repulsion of the cell membrane.

BiCellFectamax Transfection Reagent exploited recent improvements in lipofection by modifying liposome surface chemistry with polyethylene glycol (PEG). PEG coating offers several advantages to lipofection. First, PEGylated liposomes demonstrate greater transfection efficiencies as compared to liposomes lacking such surface attachments. Second, PEGylated liposomes display improved stabilities in the presence of serum, which is a particularly important feature for in vivo applications. Last, PEGylated liposomes are less likely to aggregate or form multilamellar vesicles that are morphological evolution due to vesicle-to-vesicle collision.

Sample type: Plasmid DNA or siRNA

Cell culture format: Adherent or suspension

Purpose of use: In vitro transfection

Storage buffer: lipid-aqueous solution

Storage condition: 4°C


For Research Use Only. Not for use in clinical diagnostics.

Protocol is given to demonstrate transfection in 6-well plate. Transfection in other cell culture volume can be scaled up or down accordingly.

  • Adherent cells: The day before transfection, plate 0.25-1.0 x 106 cells in 3 ml of growth medium (with serum) per well so that they will be 70-85% confluent the next day.
  • Suspension cells: On the day of transfection, plate 1.0–5.0 x106 cells in 3 ml of growth medium (with serum) per well.
  • Prepare DNA-BiCellFectamax complexes as following.
  • Dilute 1 μg of DNA in 150 μl of serum-free medium. Vortex gently to mix.
  • In a separate tube, add 15 μl of BiCellFectamax into 135 μl of serum-free medium. Vortex gently to mix.
  • Combine DNA solution with BiCelFectamax solution (total volume is 300 μl). Vortex gently to mix. Incubate for 20 minutes at room temperature to allow the DNA-liposome complexes to form.
  • Add the 300 μl of DNA-BiCellFectamax complexes to each well containing cells and medium. Mix gently by rocking the plate back and forth.
  • Incubate the cells at 37°C in a CO2 incubator for 24-72 hours to allow transgene expression.

Additional information

Volume

0.5 ml, 1 ml, 2 ml, 5 ml, 10 ml

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Be the first to review “BiCellFectamax Transfection Reagent”

"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

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