Chimeric antigen receptor T cells (also known as CAR T cells) are T cells that have been genetically engineered to produce an artificial T cell receptor for use in immunotherapy.
The extracellular domain of CAR consists in the single chain variable region moiety (scFv) from a monoclonal antibody (clone: 1D3; Rat IgG2a) raised against mouse CD19 protein. The cytoplasmic domain of CAR consists in a portion of the murine CD28 molecule from amino acids IEFMY to the 3′- terminus and the cytoplasmic region of the murine CD3ζ molecule from amino acids RAKFS to the 3′-terminus.
BiCell Scientific’s CAR-T transfection virus is used to deliver the chimeric antigen receptor of mCD19-1D3-scFv-CD28-CD3 into primary T cells that are cultured in vitro or ex vivo. The CAR-T transfection virus also expresses the GFP marker protein to facilitate FACS sorting applications.
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