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Centrin-2 antibody

$99.00$295.00

Item Cat No.: 01062

Antibody: Rat Centrin-2 Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

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Anti-Centrin-2 antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Centrin-2 (CETN2) is encoded by the CETN2 gene in human. It belongs to the Centrin family of proteins and is a structural component of the centrosome. The high level of conservation of Centrin-2 from algae to human and its association with the centrosome suggest that centrin-2 plays a fundamental role in the structure and function of the microtubule-organizing center and the proper duplication and segregation of the centrosome.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (18-aa) derived from the C-terminal region of mouse Centrin-2 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 94.4% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C


For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Centrosome (embryo section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Blocking buffer Expand NaCl 8 g KCl 0.2 g […]

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Protocol for Staining Centrosome (retina section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBS NaCl 8 g […]

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