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CEP97 antibody

$99.00$295.00

Item Cat No.: 01081

Antibody: Rabbit CEP97 Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

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Anti-CEP97 antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Centrosomal protein of 97 kDa (CEP97), also known as leucine-rich repeat and IQ domain-containing protein 2 (LRRIQ2), is encoded by the CEP97 gene in human. CEP97 acts as a key negative regulator of ciliogenesis by capping the mother centriole thereby preventing cilia formation. CEP97 is also required for recruitment of CCP110 to the centrosome.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (15-aa) derived from the N-terminal region of mouse CEP97 protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 86.7% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C


For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Centrosome (embryo section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Blocking buffer Expand NaCl 8 g KCl 0.2 g […]

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Protocol for Staining Centrosome (retina section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBS NaCl 8 g […]

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