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Fluorescent TUNEL assay kit


Item Cat No.: BCTUNL

Application: Labeling apoptotic cells

Reactivity: Tissue and Cell culture

Fluorescent TUNEL assay kit allows in situ detection and quantification of apoptosis at single cell level on cryosection or FFPE section of human and rodent tissues.

1 Kit (50 slides)

A landmark of apoptosis is the activation of nucleases that degrade the nuclear DNA into fragments of approximately 200 base pairs in length. The method of labeling DNA breaks is referred to as Terminal Deoxynucleotide Transferase dUTP Nick End Labeling, or TUNEL.

The Fluorescent TUNEL assay kit detects the DNA fragmentation of apoptotic cells by exploiting the fact that the DNA breaks expose a large number of 3′-hydroxyl ends. These hydroxyl groups can then be fluorescently labeled by terminal deoxynucleotidyl transferase (TdT), which adds deoxyribonucleotides, including FITC-dUTP, in a template-independent fashion.

The kit contains:

  • 250 ml of Universal antigen retrieval buffer (proprietary)
  • 500 μl of TUNEL Enzyme solution
  • 4.5 ml of TUNEL Label Solution (Fluorescein-dUTP, unlabeled dNTP, potassium cacodylate, CoCl2 and Tris buffered saline)
  • 250 ml of Permeabilization buffer (Tween-20, NaCl and Tris buffered saline)
  • 1 ml of Mounting buffer (Mowiol and Tris buffered saline)

Store at -20oC

For Research Use Only. Not for use in clinical diagnostics.

  • Thaw kit content to room temperature.
  • Deparaffinize FFPE sections with Xylene.
  • Rehydrate sections with decreasing concentrations of ethyl alcohol (100% to 70%).
  • Wash slides 2 times with dH2O.
  • Dip slides into universal antigen retrieval buffer.
  • Microwave sections at boiling temperature for 10min.
  • Wash slides 2 times with dH2O.
  • Dip slides into permeabilization buffer for 20min
  • Wash slides with 1xPBS
  • Drain Wash solution against a towel and put slides into the wet chamber.
  • Add 50 μl of TUNEL Enzyme solution to 450 μl of TUNEL Label Solution to obtain 500 μl of TUNEL reaction mixture. Use 100 μl of TUNEL reaction mixture per slide.
  • Incubate the slides in the wet chamber at 37oC for 1hr.
  • Take slides out of the wet chamber, drain off reaction mixture, and wash three times with 1xPBS.
  • Add 20 μl of Mowiol solution to the section on each slide. Place a cover glass onto the section and apply a small pressure to remove any trapped air bubble. Let Mowiol solution dry at room temperature for 1hr. Store glass slides at 4oC for less than 1 month.


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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