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Preactivated Fluorophore & Enzyme Conjugation Kit


Item Cat No.: BCCONJ

Concentration: 1 mg/ml of purified protein

Application: Recombinant Protein Conjugation

Reactivity: Human, Insect, Yeast

BiCell Scientific Inc has developed a series of preactivated protein conjugation kits allowing rapid conjugation of recombinant proteins as well as antibodies to fluorophore or reporter enzyme.

BiCell Scientific’s protein conjugation kit utilized the preactivated succinimidyl ester to conjugate fluorophore or reporter enzyme to target proteins (such as antibodies) that possess exposed amino groups (e.g. N-terminal amino group or ε-amino group). The reaction takes place at pH 9.0 and within concentration range of 0.5-1.0 mg/ml.

Each kit contains 10 pre-packed desalting columns and a full set of buffers to complete the conjugation and purification processes of 5 independent proteins. The kit is able to achieve conjugation on >90% available amino groups in a target protein.

Each kit contains columns, reagents and buffers sufficient for 5 independent conjugation reactions.

  • Preactivated conjugation moiety (0.5 ml)
  • 10 desalting columns (bed volume: 1 ml)
  • Activation buffer (50 ml) (Sodium bicarbonate buffered saline, pH 9.0)
  • Stop buffer (50 ml) (Tris buffered saline, pH 7.5)

Store at 4oC.

For Research Use Only. Not for use in clinical diagnostics.

Step 1. Activation of target protein

  • Centrifuge desalting column at 1,000 xg for 5min;
  • Add 1ml of Activation buffer and centrifuge at 1,000 xg for 5min, and repeat 3 times;
  • Add 1ml of target protein (0.5-1.0 mg/ml) and centrifuge at 1,000 xg for 5min;
  • Collect elutant.

Step 2. Conjugation reaction

  • Add 100μl of preactivated conjugation moiety to the eluted target protein solution;
  • Rotate at room temperature for 30min.

Step 3. Purification of conjugated protein

  • Centrifuge desalting column at 1,000 xg for 5min;
  • Add 1ml of Stop buffer and centrifuge at 1,000 xg for 5min, and repeat 3 times;
  • Add the conjugation reaction solution and centrifuge at 1,000 xg for 5min;
  • Collect elutant.

Additional information

Conjugation labels

Alexa Fluor 488, Alexa Fluor 594, Alexa Fluor 647, Horseradish Peroxidase, Alkaline Phosphatase


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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