Oncoretroviruses (cancer-causing retroviruses), include human T-lymphotropic virus (HTLV) causing a type of leukemia in humans, and murine leukemia viruses (MLVs) in mice, are powerful tools of gene transfer. The integration of oncoretroviral genome into host cells occurs during cell division, therefore, oncoretrovirus can only transduce dividing cells, but not cells that have entered terminal differentiation, such as neurons.
Oncoretroviruses have gag, pol and env genes, coding for viral proteins in the order: 5´-gag–pol–env-3´. The 5′-end of long terminal repeat (LTR) includes four regions, which are R, U5, PBS (primer binding site), and L; the 3′-end of LTR includes three regions, which are PPT (polypurine tract), U3, and R.
BiCell Scientific’s retrovirus packaging kit provides all the components needed to generate retrovirus, including expression vectors, helper vectors, HEK293T cell line, and BiCellFectamax transfection reagent.
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