Protocol for Making Monoclonal Antibody

Preparation of Myeloma Cells Expand Prewarm all culture media to 37oC. Thaw the parental myeloma cells (Sp2/0-Ag14 or X63-Ag8.653) from liquid nitrogen and culture in BiCell-mAb Medium MY for 3-5 days to fully recover cell growth to reach 1 x 106/ml cell density. Split cells at 1:5 into fresh BiCell-mAb Medium MY and let grow […]

Read More »