Peptide tags can be appended to proteins so that they can be purified from their crude biological sources using affinity chromatography. Recombinant protein expression system such as E. coli, when combined with the usage of peptide tags, offers a quick and cheap solution to large-scale manufacturing of protein biologics.
Bacterial expression system, however, has limitations in recombinant protein folding, which are largely caused by the lack of eukaryotic protein translation machinery. BiCell Scientific Inc has developed a variety of robust protein expression systems utilizing eukaryotic cell models, e.g. human HEK293 cells (or industrial-grade CHO cells), insect Sf9 cells and yeast Pichia pastoris cells. Such expression systems can achieve recombinant protein yield ranging from 1-10 mg/ml.
Reviews
There are no reviews yet.