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Tagged Recombinant Protein Production


Concentration: 1 mg/ml of purified protein

Application: Recombinant Protein Expression

Reactivity: Human, Insect, Yeast

BiCell Scientific Inc has developed a robust protein expression system utilizing short peptide tags to purify recombinant proteins from a variety of model cells.

Peptide tags can be appended to proteins so that they can be purified from their crude biological sources using affinity chromatography. Recombinant protein expression system such as E. coli, when combined with the usage of peptide tags, offers a quick and cheap solution to large-scale manufacturing of protein biologics.

Bacterial expression system, however, has limitations in recombinant protein folding, which are largely caused by the lack of eukaryotic protein translation machinery. BiCell Scientific Inc has developed a variety of robust protein expression systems utilizing eukaryotic cell models, e.g. human HEK293 cells (or industrial-grade CHO cells), insect Sf9 cells and yeast Pichia pastoris cells. Such expression systems can achieve recombinant protein yield ranging from 1-10 mg/ml.

  • Gene synthesis of cDNA encoding target protein
  • Molecular cloning of cDNA into lentivirus, baculovirus or yeast targeting vector
  • Infection of HEK293 cells or CHO cells or Sf9 cells or DNA recombination in Pichia yeast
  • Validation of recombinant protein expression in host cells by Western blot
  • Selection and expansion of the highest expressing clone
  • Purification of recombinant protein by affinity chromatography and size exclusion chromatography
  • Affinity purified recombinant protein (1 mg)
  • Model cells stably expressing recombinant protein (frozen HEK293 or CHO or Sf9 or Pichia yeast, 1×106 cells)


For Research Use Only. Not for use in clinical diagnostics.

Additional information

Protein Expression System

Industrial-grade CHO cell, Human HEK293 cell, Insect Sf9 cell, Yeast Pichia pastoris

Tag Purification System

His-tag, Flag-tag, Myc-tag, HA-tag, GST-tag


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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

School of Medicine | Cleveland Clinic

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