Western Blot detects denatured and linearized proteins in SDS buffers. Conventional antibodies raised against epitopes assuming natural folding conformations are not suited for Western Blot. When used for Western Blot, conventional antibodies often report multiple bands of different molecular weights but only one band being specific binding.
BiCell Scientific Inc has developed a novel denaturing and stabilizing technique to prevent the natural folding of epitope sequence in order to lock the conformational state of epitope in unfolded state. BiCell Scientific Inc is able to raise polyclonal and monoclonal antibodies against the unfolded target epitope. Such antibodies can dramatically improve the binding specificity on Western Blot, which will be vital tools of quantitative measurements of protein expression in endogenous cell or tissue samples.
Reviews
There are no reviews yet.