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ZO-1 (Tjp1) (C-terminus) monoclonal antibody labeling of embryonic mouse liver

ZO-1 (Tjp1) (C-terminus) monoclonal antibody labeling of embryonic mouse liver

ZO-1 proteins are found in the cell junction of the bile canaliculus of the liver from a mouse embryo at E15.5. p.v., portal vein.

ZO-1 in embryonic mouse lung

ZO-1 proteins are found in the cell junctions of primordial alveolar epithelium in the lung of a mouse embryo at E15.5. B., bronchiole.

ZO-1 (Tjp1) (C-terminus) monoclonal antibody (F11 clone)

$245.00 – $585.00

Item Cat No.: 00236f11

Antibody: Rat Anti-ZO-1 (Tjp1) (C-terminus) monoclonal antibody

Concentration: Purified IgG - 0.25 mg/ml

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, Mouse, Rat

Anti-ZO-1 (Tjp1) (C-terminus) monoclonal antibody (F11 clone) is validated on mouse and human tissues and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

Product price: $245.00
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Anti-ZO-1 (Tjp1) (C-terminus) monoclonal antibody (F11 clone) is validated on mouse and human tissues and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Zonula occludens-1 (ZO-1), also known as tight junction protein-1 (TJP1) is a 220-kD tight junction peripheral protein encoded by the TJP1 gene in human. ZO-1 contains three PDZ domains, an SRC homology 3 (SH3) domain, and a yeast guanylate kinase homology (GUK) domain. ZO-1 can interact with tight junction integral proteins such as claudins and occludin and with the perijunctional cytoskeleton.

ZO-1 is expressed in a variety of epithelial tissues including the kidney, the intestines, the lung, the liver, the pancreas and more.

Host/Isotype: Rat/IgG

Class: Monoclonal

Immunogen: Synthetic peptide (17-aa) derived from the C-terminal region of human ZO-1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

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