Protocol for Mouse ES Cell Culture

Gelatin coating of culture dish Expand 1. Coat dish (35-mm to 150-mm diameter) by adding 500μl – 5ml ready-to-use gelatin solution to cover the bottom; rock the dish back and forth to let solution cover homogeneously; sit at room temperature for 3hr (keep sterile in culture hood). 2. Aspirate solution; allow air dry overnight in culture hood […]

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