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FAT1 antibody labeling of embryonic mouse kidney

FAT1 antibody labeling of embryonic mouse kidney

FFPE section of mouse embryo shows FAT1 protein localization in the luminal membrane of the epithelium making the primordial tubules in the kidney.

FAT1 antibody labeling of mouse retina

FAT1 antibody labeling of mouse retina

FAT1 proteins are found in the external limiting membrane, where the photoreceptor cells make cell junctions with the muller cells, and the synapses in the outer plexiform layer (OPL) of the mouse retina, where the bipolar cells make contact with the photoreceptor cells. ONL: outer nuclear layer; INL: inner nuclear layer.

FAT1 in the developing skin

FAT1 proteins are found in the cell junctions made by the stratified epithelium in the epidermis from an embryonic mouse at E15.5.

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FAT1 antibody

$245.00 – $585.00

See High Resolution Histopathology Image

Item Cat No.: 50501

Antibody: Rabbit FAT1 Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-FAT1 antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Optional Blocking Peptide

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

Product price: $245.00
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Anti-FAT1 antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Protocadherin FAT1 is a protein that is encoded by the FAT1 gene in human. This gene is an orthologue of the Drosophila fat gene. FAT is involved in establishing the planar polarity in Drosophila cells.

Like classic cadherins, protocadherin proteins contain an amino-terminal extracellular domain or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain. Compared to classic cadherins, the ectodomains in protocadherins are much larger.

FAT1 is highly expressed in the external limiting membrane of the retina, where the photoreceptor cells make cell junctions with the muller cells.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (14-aa) derived from the C-terminal region of human FAT1 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Protocadherin (embryo section)

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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Protocol for Staining FAT1

General and Specific Protocols

Materials and Reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

"The rabbit hybridoma supernatants of anti-APOBEC3 project are tested positive by ELISA, and we are very happy about it! We previously tried a company, Abclone. Their Project "A" has immune response that is <10,000 titer in antiserum, which would explain why there is no positive mAb after fusion. Their project "B" didn't have any immune response in rabbit."

Harshita B Gupta, PhD.

School of Medicine | UT Health San Antonio

"We have tested anti mouse T cell antiserum samples from both rabbits you sent to us.

They worked very well! Thank you!"

Victoria Gorbacheva, PhD.

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