Frizzled-7 (FZD7) antibody labeling of embryonic mouse brain

Frizzled-7 proteins are found in the developing neurons of the forebrain from a mouse embryo at E17.5.

Frizzled-7 in forebrain white matter

Frizzled-7 proteins are found in the nerve fibers of the white matter in the forebrain from a mouse embryo at E17.5.

Frizzled-7 in the CNS nerve

Frizzled-7 proteins are found in the nerve fibers of the white matter in the hindbrain from a mouse embryo at E17.5.

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Frizzled-7 (FZD7) antibody

Name: Frizzled-7 (FZD7) antibody
SKU: 50607
Availability: InStock

$245.00 – $585.00

Item Cat No.: 50607

Antibody: Rat polyclonal Frizzled-7 (FZD7) antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-Frizzled-7 (FZD7) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

volume

Clear

Optional Blocking Peptide

No blocker

1 ug blocker (+$69.00)

10 ug blocker (+$119.00)

FFPE Specific Antibody

FFPE-specific recognition antibodies are made against the same epitope sequence with novel cross-linking and stabilizing technique to lock the conformational state of epitope in a folded state similar to aldehyde induced fixation.

No FFPE Specific Antibody Antibody Add-on

200 ul FFPE Specific Antibody Antibody (+$475.00)

50 ul FFPE Specific Antibody Antibody (+$195.00)

Western Blot Specific Antibody

Western blot-specific recognition antibodies are made against the same epitope sequence with novel denaturing and stabilizing technique to prevent the natural folding of epitope in order to lock its conformation in unfolded state.

No western blot add-on

200 ul Western Blot Specific Antibody (+$475.00)

50 ul Western Blot Specific Antibody (+$195.00)

Product price:	$245.00
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Anti-Frizzled-7 (FZD7) antibody is validated on mouse tissue and can be used for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Frizzled class receptor 7, Frizzled-7 is encoded by the FZD7 gene in human. Frizzled-7 consists in a cysteine-rich extracellular domain of Fz family members, 7 transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif.

Frizzled-7 can interact with DLG4 and mediate both canonical and non-canonical Wnt responses. Frizzled-7 also participates in planar polarity information during tissue morphogenesis.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (16-aa) derived from the N-terminal extracellular region of human frizzled-7 protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Frizzled-2 to -7 (retina section)

General and Specific Protocols

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBSNaCl 8 gKCl 0.2 gNa2HPO4 1.44 […]

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Frizzled-7 (FZD7) antibody

Optional Blocking Peptide

FFPE Specific Antibody

Western Blot Specific Antibody

Protocol for Staining Frizzled-2 to -7 (retina section)

Reviews

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