Protocol for Staining Frizzled-2 to -7 (retina section)

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBSNaCl 8 gKCl 0.2 gNa2HPO4 1.44 […]

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