In situ hybridization enables the detection and precise localization of a specific nucleic acid sequence within an individual cell. The nucleic acid sequence is bound specifically in a tissue section by complementary base pairing, that is, hybridization, with a detectable nucleic acid segment called a probe.
BiCell Scientific Inc has developed and validated an in situ hybridization kit for microRNA detection based upon DIG-labeled oligonucleotide probe. The kit can be used for both cryosections and FFPE sections of rodent or human tissues. The oligonucleotide is made of locked nucleic acid to enhance binding affinity with microRNA molecule. Each kit includes a validated oligonucleotide probe that has been selected for the target gene.
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