Nephrocystin-5 (NPHP5 or IQCB1) antibody labeling of mouse retina

Nephrocystin-5 proteins are localized to the connecting cilium (in IS and ONL layers) in the photoreceptor cells in the mouse retina, which is equivalent to the primary cilium in the epithelium. IS: inner segment of photoreceptor cell layer; ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer.

Nephrocystin-5 in the kidney

Nephrocystin-5 proteins are localized to the primary cilia in the epithelial cells of mouse kidney tubules. Cell junctions are counter-stained with ZO-1.

Nephrocystin-5 in the inner ear

Nephrocystin-5 proteins are found in the primary cilia of the hair cells in the organ of Corti in the mouse inner ear. Phalloidin decorates the actin-rich stereocilia and the cuticular plate of the hair cells.

NPHP5 in P10 mouse retina

Courtesy of Dr. Abigail Moye (Baylor College of Medicine)