Wheat Germ Agglutinin (WGA) Fluorophore Conjugate labeling polarized MDCK-II cells
WGA-Rhodamine (red) labels the luminal membrane while ER-binder (green) labels the endoplasmic reticulum of the MDCK-II cells cultured on Transwell inserts. Top panel: sagittal view of the MDCK-II cell monolayer. Bottom panel: transverse view of MDCK-II cell monolayer sliced at vertical position of a and b.
$175.00
200 µl size
Wheat Germ Agglutinin (WGA) Fluorophore Conjugate labels the N‐acetylglucosamine and N‐acetylneuraminic acid (sialic acid) residues of the proteins in the plasma membrane of mammalian cells.
Wheat germ agglutinin (WGA) is a lectin protein that binds to N-acetyl-D-glucosamine and Sialic acid on the residues of the proteins located in the plasma membrane of mammalian cells. WGA exists mostly as a heterodimer of 38 kDa. It is cationic at physiological pH.
Because the glycoconjugates are extracellular modifications, WGA can be used to stain live cell cultures of human and rodent origins.
Conjugation: Fluorophore conjugated
Purification: Affinity chromatography
Storage buffer: PBS, pH 7.2, 0.1% sodium azide
Storage condition: –20°C
For Research Use Only. Not for use in clinical diagnostics.
Staining of cell culture
| Fluorescent labels | FITC, Rhodamine |
|---|
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