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Centriolin (CEP110) antibody

$99.00$295.00

Item Cat No.: 01083

Antibody: Rat Centriolin (CEP110) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

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Anti-Centriolin (CEP110) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

Centriolin (CNTRL), also known as CEP110, is encoded by the CNTRL gene in human. Centriolin is a centrosomal protein required for the centrosome to function as a microtubule organizing center. Centriolin is also involved in cell cycle progression and cytokinesis. During the late step of cytokinesis, Centriolin anchors exocyst and SNARE complexes at the midbody, thereby allowing secretory vesicle-mediated abscission.

Host/Isotype: Rat/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (17-aa) derived from the N-terminal region of mouse Centriolin protein

Species homology of immunogen: Synthetic peptide sequence is identical to rat sequence (showing 70.6% homology to human sequence)

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C


For Research Use Only. Not for use in clinical diagnostics.

Protocol for Staining Centrosome (embryo section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Blocking buffer Expand NaCl 8 g KCl 0.2 g […]

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Protocol for Staining Centrosome (retina section)

Materials and reagents Expand Leica CM1950 automatic sectioning cryostat. Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5). Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR. Tissue-tek cryomolds from VWR. Buffers Expand 0.5% paraformaldehyde in PBS NaCl 8 g […]

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