RNA interference (RNAi) is a biological process in which a small fragment of double-stranded RNA (dsRNA) molecule (19-22 nt long) exerts sequence-specific suppression of gene expression, known as gene silencing. Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of dsRNA consisting in phosphorylated 5′ ends and hydroxylated 3′ ends, as well as two overhanging Uracil nucleotides.

BiCell Scientific’s siRNA synthesis and validation service provides customized siRNA design, synthesis and validation services to make pre-annealed and ready-to-use siRNA molecules for gene silencing experiments both in vitro and in vivo.

BiCell Scientific’s in vitro grade siRNA synthesis incorporates 2′-O-Methyl (2′-OMe) modification to the nucleotides of the antisense strand, which can significantly enhance the resistance of antisense strand to degradation by Ribonuclease H. In vitro transfection of siRNA is recommended using BiCellFectamax Transfection Reagent.

BiCell Scientific’s in vivo grade siRNA synthesis not only incorporates 2′-O-Methyl (2′-OMe) modification to the antisense strand, but also conjugates cholesterol to its 3′ end, which can significantly enhance the delivery of siRNA to various extrahepatic organs such as tumors, placenta, muscles, heart and lung. In vivo transfection of siRNA is recommended using BiCellFectamaxVivo Transfection Reagent.