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VE-cadherin (Cdh5 or Cadherin-5 or CD144) antibody

(1 customer review)


Item Cat No.: 00105

Antibody: Rabbit VE-cadherin (Cdh5 or Cadherin-5 or CD144) Polyclonal Antibody

Concentration: 0.25 mg/ml purified IgG

Application: Validated by immunofluorescence labeling (1:100)

Reactivity: Human, mouse, rat

Anti-VE-cadherin (Cdh5 or Cadherin-5 or CD144) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

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Anti-VE-cadherin (Cdh5 or Cadherin-5 or CD144) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues.

VE-cadherin is a protein that is encoded by the Cdh5 gene in human. VE-cadherin is also known as Cadherin-5 or CD144. VE-cadherin belongs to the classic cadherin protein superfamily.

The cadherin protein features an amino-terminal extracellular domain or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain. The cadherin ectodomain plays an adhesive role whereas the intracellular domain mediates cadherin-catenin interactions.

VE-cadherin is exclusively expressed in the endothelium. VE-cadherin is important for the integrity of adherens junction in the endothelium and the permeability across the endothelium.

Host/Isotype: Rabbit/IgG

Class: Polyclonal

Immunogen: Synthetic peptide (13-aa) derived from the C-terminal region of human VE-cadherin protein

Species homology of immunogen: Synthetic peptide sequence is identical to mouse or rat sequence

Conjugation: Unconjugated

Purification: Affinity chromatography

Storage buffer: PBS, pH 7.2, 0.1% sodium azide

Storage condition: –20°C

For Research Use Only. Not for use in clinical diagnostics.

Annotation of mouse endothelia

The skin Expand The skin, or integument, consists of two major layers: the epidermis, composed of stratified squamous epithelium that is keratinized, and the dermis, composed of connective tissue. Figure legend VE-cadherin proteins label the endothelial cell junctions of the capillaries in the dermis of the skin from a mouse embryo at E15.5. Ep, epidermis; […]

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Protocol for Staining Cadherin

Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass.   Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […]

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1 review for VE-cadherin (Cdh5 or Cadherin-5 or CD144) antibody



    I am vikram subramanian, postdoc research fellow at university of Iowa. I have recently purchased VE-cadherin antibody from bicellscientific. It worked very good for western blotting also, a clear single band. I should have found this antibody earlier before all struggles but now i am happy it worked perfectly. The best antibody for VE-Cadherin. no second thought go for it.

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"I am really impressed with your approach. We tried multiple times previously to create monoclonal and polyclonal antibodies to claudin-2 and MLCK1. We have had limited success generating polyclonals and no success generating monoclonals. You have generated outstanding monoclonals to both. I look forward to continuing to work with you."

Jerrold R. Turner, M.D., Ph.D.

Brigham and Women’s Hospital | Harvard Medical School

"The polyclonal antibody you generated for KIAA0408 is stunning! KIAA0408 is a novel cilium molecule that has never been studied. So, clearly there will be a lot of demand for it as we have discovered a very interesting finding and the story will be published in a high impact journal. I am strongly inclined to generate monoclonal antibody for this protein too and we should think about patenting it."

Univ.-Prof. Jay Gopalakrishnan PhD

Heinrich-Heine-Universität | Universitätsklinikum Düsseldorf

"Your ARL13B antibody works beautifully!!! We’re so happy to have a cilia-specific antibody made in rat! I can send you high resolution images to be posted on your website."

Julie Craft Van De Weghe, PhD

School of Medicine | University of Washington

"The assay is a homophilic interaction mediated cell adhesion on purified protein (in this case, immobilized purified Pcdhga9 to Pcdhga9 expressed on cell surface). Compared to control, cell adhesion is reduced in the presence of Pcdhga9 monoclonal antibody supernatants!"

Divyesh Joshi, PhD

School of Medicine | Yale University

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